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Breaking Cas
Breaking Cas enables users to design sgRNAs using from all the genomes available on the ENSEMBL database.
![](https://www.metabion.com/uploads/breaking-cas.png)
Spanish National Biotechnology Centre (Centro Nacional de Biotecnología, CNB - Consejo Superior de Investigaciones Científicas, CSIC)
Designing your sgRNA is a key step towards a successful CRISPR-Cas genome editing experiment. There a number of online tools dedicated to designing sgRNAs. One of the latest and most complete tool is Breaking Cas.
For first-time users, you can read our guide here.
To find out more about CRISPR-Cas, visit our CRISPR-Cas page!
References