2’-F-A

2'-Fluoro-deoxyadenosine (2'-F-A, Figure 1) confers thermodynamical stability to an oligonucleotide during hybridization of the oligo with its target. When incorporated into an antisense, the duplex Tm increases by 1.8 °C per 2'-F residue added, compared to the native antisense. In RNA oligos, it is suggestable to additionally phosphorothiolate the 2'-F RNA phosphodiester linkages, to increase the oligo resistance to RNase H.

Figure 1. Structure of 2’-F-A (R = sequence).

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References

Layzer JM., McCaffrey AP., Tanner AK., Huang Z., Kay MA., Sullenger BA. In vivo activity of nuclease-resistant siRNAs. RNA. 2004 May;10(5):766-71.