Atto 425 dye presents a strong absorption capability, along with high photo-stability and moderate hydrophilicity. This dye is also characterized by a high fluorescence quantum yield, which is referred as the ratio between absorbed photons and emitted fluorescence photons. It presents a coumarin structure, and it is usually coupled to DNA and RNA oligonucleotides.
Figure 1. Structure of 5'-Atto 425 (R = sequence).
metabion offers Atto 425 (Figure 1) in its DNA and RNA portfolio as a 5'-, 3'- or internal Atto 425-dT modification: