6-Fam Azide
6-Fam (6-carboxyfluorescein) is one single isomer of the dye Fluorescein. The spectral properties of 6-Fam are identical to those of FITC (Fluorescein-5,6-isothiocyanate), which consists of a reactive specie of Fluorescein bearing an isothiocyanate reactive group. 6-Fam can be successfully used with most fluorescent detection devices available in the market. 6-Fam-Azid is an azide modifier that can be used in Click-Chemistry. This technique allows multiple alkynated multiple sites within an oligonucleotide to be labelled by “clicking” a variety of azide-containing labels/tags. To learn more about click-chemistry, click here.
Metabion offers 6-Fam Azide as a modification in its click-chemistry portfolio. Click here
| 6-Fam Azide specifications | |
|---|---|
| MW | 458.83 g/mol |
| Excitation maximum | 496 nm |
| Emission maximum | 516 nm |
| Extinction coefficient (260nm) | 83 mM-1cm-1 |