6-Fam
6-Fam (6-carboxyfluorescein) is one single isomer of the dye Fluorescein. It is usually coupled to DNA and RNA oligonucleotides via an amide bond and it has a good solubility in water. Spectral characteristics of 6-Fam are identical to those of FITC (Fluorescein-5,6-isothiocyanate), which consists of a reactive species of fluorescein bearing an isothiocyanate reactive group. 6-Fam can be successfully used with most fluorescent detection devices available in the market.
metabion offers 6-Fam modifications in its DNA and RNA portfolio. Click here
| 6-Fam as 5' modification | |
|---|---|
| MW | 538 g/mol |
| Excitation maximum | 495 nm |
| Emission maximum | 520 nm |
| Extinction coefficient (260nm) | 20.9 mM-1cm-1 |
| 6-Fam as 3' modification | |
|---|---|
| MW | 598 g/mol |
| Excitation maximum | 495 nm |
| Emission maximum | 520 nm |
| Extinction coefficient (260nm) | 20.9 mM-1cm-1 |
| 6-Fam as internal 6-Fam-dT | |
|---|---|
| MW | 852 g/mol |
| Excitation maximum | 495 nm |
| Emission maximum | 520 nm |
| Extinction coefficient (260nm) | 30.2 mM-1cm-1 |
K.J. Livak, S.J.A. Flood, J. Marmaro, W. Giusti, and K. Deetz. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. (1995), 4: 1-6