Atto 425
Atto 425 dye presents a strong absorption capability, along with high photo-stability and moderate hydrophilicity. This dye is also characterized by a high fluorescence quantum yield, which is referred as the ratio between absorbed photons and emitted fluorescence photons. It presents a coumarin structure, and it is usually coupled to DNA and RNA oligonucleotides.
Metabion offers the Atto 425 modification in its DNA and RNA portfolio. Click here
| Atto 425 as a 5' modification | |
|---|---|
| MW | 563 g/mol |
| Excitation maximum | 439 nm |
| Emission maximum | 485 nm |
| Extinction coefficient (260nm) | 12.1 mM-1cm-1 |
| Atto 425 as a 3' modification | |
|---|---|
| MW | 593 g/mol |
| Excitation maximum | 439 nm |
| Emission maximum | 485 nm |
| Extinction coefficient (260nm) | 12.1 mM-1cm-1 |
| Atto 425 as an internal Atto 425-dT | |
|---|---|
| MW | 786 g/mol |
| Excitation maximum | 439 nm |
| Emission maximum | 485 nm |
| Extinction coefficient (260nm) | 12.4 mM-1cm-1 |