Atto 647N

Atto 647N presents strong absorption and high quantum yield, which is referred as the ratio between absorbed photons and emitted fluorescence photons. This dye consists of 2 isomers bearing identical absorbance and fluorescence. It is usually coupled to DNA and RNA oligonucleotides and it has a good photo-stability.

Metabion offers the Atto 647N modification in its DNA and RNA portfolio. Click here

Atto 647N as a 5' modification
MW 1006 g/mol
Excitation maximum 646 nm
Emission maximum 664 nm
Extinction coefficient (260nm) 9 mM-1cm-1
Atto 647N as a 3' modification
MW 1180 g/mol
Excitation maximum 646 nm
Emission maximum 664 nm
Extinction coefficient (260nm) 9 mM-1cm-1
Atto 647N as an internal Atto 647N-dT
MW 1240 g/mol
Excitation maximum 646 nm
Emission maximum 664 nm
Extinction coefficient (260nm) 18.3 mM-1cm-1

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