Atto 647N
Atto 647N presents strong absorption and high quantum yield, which is referred as the ratio between absorbed photons and emitted fluorescence photons. This dye consists of 2 isomers bearing identical absorbance and fluorescence. It is usually coupled to DNA and RNA oligonucleotides and it has a good photo-stability.
Metabion offers the Atto 647N modification in its DNA and RNA portfolio. Click here
| Atto 647N as a 5' modification |
|---|
| MW |
1006 g/mol |
| Excitation maximum |
646 nm |
| Emission maximum |
664 nm |
| Extinction coefficient (260nm) |
9 mM-1cm-1 |
| Atto 647N as a 3' modification |
|---|
| MW |
1180 g/mol |
| Excitation maximum |
646 nm |
| Emission maximum |
664 nm |
| Extinction coefficient (260nm) |
9 mM-1cm-1 |
| Atto 647N as an internal Atto 647N-dT |
|---|
| MW |
1240 g/mol |
| Excitation maximum |
646 nm |
| Emission maximum |
664 nm |
| Extinction coefficient (260nm) |
18.3 mM-1cm-1 |
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