Cy3B
Cy3B represents one isomer of the dye Cy3. As such, it does not incur into photo-isomerization events, and it is characterized by high stability and high degree of fluorescence emission.
Metabion offers the Cy3B modification in its DNA portfolio. Click here
| Cy3B as a 5' modification | |
|---|---|
| MW | 722 g/mol |
| Excitation maximum | 559 nm |
| Emission maximum | 570 nm |
| Extinction coefficient (260nm) | 4.9 mM-1cm-1 |
| Cy3B as a 3' modification | |
|---|---|
| MW | 752 g/mol |
| Excitation maximum | 559 nm |
| Emission maximum | 570 nm |
| Extinction coefficient (260nm) | 4.9 mM-1cm-1 |
| Cy3B as an internal Cy3B-dT | |
|---|---|
| MW | 945 g/mol |
| Excitation maximum | 559 nm |
| Emission maximum | 570 nm |
| Extinction coefficient (260nm) | 14.2 mM-1cm-1 |
M. Cooper, A. Ebner, M. Briggs, M. Burrows, N. Gardner, R. Richardson, and R. West. Cy3B: improving the performance of cyanine dyes. J. Fluoresc. (2004), 14: 1145-150