Joe
The chemical name of the fluorescent dye Joe is 4,5-Dichloro-Dimethoxy-Fluorescein. Joe derives from Fluorescein and can be used as an alternative to VIC®, due to their similar spectral properties. It is often used in multiplex PCR in combination with FAM.
Metabion offers Joe as a modification in its DNA and RNA portfolio. Click here
| Joe as a 5' modification | |
|---|---|
| MW | 667 g/mol |
| Excitation maximum | 522 nm |
| Emission maximum | 548 nm |
| Extinction coefficient (260nm) | 12 mM-1cm-1 |
| Joe as a 3' modification | |
|---|---|
| MW | 697 g/mol |
| Excitation maximum | 522 nm |
| Emission maximum | 548 nm |
| Extinction coefficient (260nm) | 12 mM-1cm-1 |
| Joe as an internal modification | |
|---|---|
| MW | 981 g/mol |
| Excitation maximum | 522 nm |
| Emission maximum | 548 nm |
| Extinction coefficient (260nm) | 21.3 mM-1cm-1 |