Rox
The chemical name of Rox is 6-Carboxyl-X-Rhodamine. This dye is normally used as a reference dye to normalize the fluorescence signal arising from fluorescent probes in real-time PCR applications. Rox represents an excellent alternative to PET®, due to their similar spectral properties.
Metabion offers Rox as a modification in its DNA and RNA portfolio. Click here
| Rox as a 5' modification | |
|---|---|
| MW | 698 g/mol |
| Excitation maximum | 576 nm |
| Emission maximum | 601 nm |
| Extinction coefficient (260nm) | 22.6 mM-1cm-1 |
| Rox as a 3' modification | |
|---|---|
| MW | 728 g/mol |
| Excitation maximum | 576 nm |
| Emission maximum | 601 nm |
| Extinction coefficient (260nm) | 22.5 mM-1cm-1 |
| Rox as an internal modification | |
|---|---|
| MW | 921 g/mol |
| Excitation maximum | 576 nm |
| Emission maximum | 601 nm |
| Extinction coefficient (260nm) | 31.8 mM-1cm-1 |