2’-Deoxyuridine can be incorporated in DNA oligonucleotides as an analog of dT. This base can be removed by treatment with uracil-N-deglycosylase (UNG), which is normally used to digest amplified DNA and eliminate DNA-RNA cross-contamination.
2’-Deoxyuridine modified oligos can be used to study adaptive and innate immunity in animals.
Metabion offers 2’-Deoxyuridine in its DNA and RNA portfolio. Click here
|Extinction coefficient (260 nm)||10 mM-1cm-1|
M.S. Neuberger, R.S. Harris, J. Di Noia, S.K. Petersen-Mahrt. Immunity through DNA deamination. Trends Biochem. Sci. (2003), 28: 305-312.
R.S. Harris, A.M. Sheehy, H.M. Craig, M.H. Malim, M.S. Neuberger. DNA deamination: not just a trigger for antibody diversification but also a mechanism for defense against retroviruses. Nature Immunology (2003), 4: 641-643.