5-F-dU can pair with purine bases (so, A and G). Importantly, the pairs A:(5-F-dU)and G:(5-F-dU) are more stable than A:T and G:T, the latter being a mismatched base pair. Thanks to this characteristic, insertion of 5-F-dU in hybridization probes allows the creation of unique, highly selective probes, which can be used for cDNA library screening.
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J.F. Habener, C.D. Vo, D.B. Le, G.P. Gryan, L. Ercolani, and A.H-J. Wang. 5-Fluorodeoxyuridine as an alternative to the synthesis of mixed hybridization probes for the detection of specific gene sequences. Proc. Natl. Acad. Sci. USA (1988), 85: 1735-1739.