5-Hydroxymethyl-C
5-hydroxymethylcytosine (5-Hydroxymethyl-C) is also known as “the sixth base”, given that it has been detected in all animal tissues, with particularly high level in promoter and intragenic regions. 5-hydroxymethylcytosine derives from 5-mC, which plays an important role as an epigenetic marker in gene expression and regulation. However, which role 5-hydroxymethylcytosine plays is still not clear. Incorporation of this base in synthetic oligonucleotides will help researchers elucidating the role of this base.
Metabion offers 5-Hydroxymethyl-C in its DNA portfolio. Click here
| Specifications | |
|---|---|
| MW | 319.2 g/mol |
| Extinction coefficient (260 nm) | 7 mM-1cm-1 |
N.W. Penn, R. Suwalski, C. O’Riley, K. Bojanowski, and R. Yura. The Presence of 5-Hydroxymethylcytosine in Animal Deoxyribonucleic Acid. Biochem. J. (1972), 126: 781-790.
H. Sasaki, and Y. Matsui. Epigenetic events in mammalian germ-cell development: reprogramming and beyond. Nat. Rev. Genet. (2008), 9: 129-140.
M. Tahiliani, K.P. Koh, Y. Shen, W.A. Pastor, H. Bandukwala, Y. Brudno, S. Agarwal, L.M. Iyer, D. Liu, L. Aravind, and A. Rao. Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1 Science (2000), 324: 930-935.