5-hydroxymethylcytosine (5-Hydroxymethyl-C) is also known as “the sixth base”, given that it has been detected in all animal tissues, with particularly high level in promoter and intragenic regions. 5-hydroxymethylcytosine derives from 5-mC, which plays an important role as an epigenetic marker in gene expression and regulation. However, which role 5-hydroxymethylcytosine plays is still not clear. Incorporation of this base in synthetic oligonucleotides will help researchers elucidating the role of this base.
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|Extinction coefficient (260 nm)||7 mM-1cm-1|
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