BHQ®-2
BHQ®-2 stands for Black Hole Quencher 2 and it consists of a dark quencher, often used in dual-labelled probes. As dark quenchers, Black Hole Quenchers absorb energy without emitting fluorescence. They are in fact non-fluorescent chromophores.
Metabion offers BHQ®-2 in its DNA and RNA dual-labelled probes portfolio. Click here
| BHQ®-2 as 5' modification | |
|---|---|
| MW as 5' | 539 g/mol |
| Absorption range | 550-650 nm |
| Absorption maximum | 579 nm |
| Extinction coefficient (260nm) | 8 mM-1cm-1 |
| BHQ®-2 as 3' modification | |
|---|---|
| MW | 554 g/mol |
| Absorption range | 550-650 nm |
| Absorption maximum | 579 nm |
| Extinction coefficient (260nm) | 8 mM-1cm-1 |
| BHQ®-2 as an internal modification | |
|---|---|
| MW | 962 g/mol |
| Absorption range | 550-650 nm |
| Absorption maximum | 579 nm |
| Extinction coefficient (260nm) | 16.4 mM-1cm-1 |