BHQ®-3
BHQ®-3 stands for Black Hole Quencher 3 and it consists of a dark quencher, often used in dual-labelled probes. As dark quenchers, Black Hole Quenchers absorb energy without emitting fluorescence. They are in fact non-fluorescent chromophores.
Metabion offers BHQ®-3 modification in its DNA portfolio. Click here
| BHQ®-3 as 5' modification | |
|---|---|
| MW as 5' | 597 g/mol |
| Absorption range | 620-730 nm |
| Absorption maximum | 672 nm |
| Extinction coefficient (260nm) | 13 mM-1cm-1 |
| BHQ®-3 as 3' modification | |
|---|---|
| MW | 598.7 g/mol |
| Absorption range | 620-730 nm |
| Absorption maximum | 672 nm |
| Extinction coefficient (260nm) | 8 mM-1cm-1 |