Dabcyl
Dabcyl is a dark quencher often used in dual-labelled probes. The 3’-terminus of Dabcyl consists of a free OH. When Dabcyl is inserted at the 3’-end of a primer/probe, the polymerase can theoretically elongate the primer/probe. However, the efficiency of this elongation is rather low.
Metabion offers Dabcyl in its DNA and RNA dual-labelled probes portfolio. Click here
| Dabcyl as 5' modification | |
|---|---|
| MW as 5' | 430.2 g/mol |
| Absorption range | 380-550 nm |
| Absorption maximum | 453 nm |
| Extinction coefficient (260nm) | 11.1 mM-1cm-1 |
| Dabcyl as 3' modification | |
|---|---|
| MW | 756 g/mol |
| Absorption range | 380-550 nm |
| Absorption maximum | 453 nm |
| Extinction coefficient (260nm) | 11.1 mM-1cm-1 |
| Dabcyl as an internal modification | |
|---|---|
| MW | 745 g/mol |
| Absorption range | 380-550 nm |
| Absorption maximum | 453 nm |
| Extinction coefficient (260nm) | 20.4 mM-1cm-1 |