Dabcyl
Dabcyl is a dark quencher often used in dual-labelled probes. The 3’-terminus of Dabcyl consists of a free OH. When Dabcyl is inserted at the 3’-end of a primer/probe, the polymerase can theoretically elongate the primer/probe. However, the efficiency of this elongation is rather low.
Metabion offers Dabcyl in its DNA and RNA dual-labelled probes portfolio. Click here
Dabcyl as 5' modification | |
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MW as 5' | 430.2 g/mol |
Absorption range | 380-550 nm |
Absorption maximum | 453 nm |
Extinction coefficient (260nm) | 11.1 mM-1cm-1 |
Dabcyl as 3' modification | |
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MW | 756 g/mol |
Absorption range | 380-550 nm |
Absorption maximum | 453 nm |
Extinction coefficient (260nm) | 11.1 mM-1cm-1 |
Dabcyl as an internal modification | |
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MW | 745 g/mol |
Absorption range | 380-550 nm |
Absorption maximum | 453 nm |
Extinction coefficient (260nm) | 20.4 mM-1cm-1 |