Eclipse®
Eclipse® is a dark quencher often used in dual-labelled probes. As dark quenchers, Eclipse® absorbs energy without emitting fluorescence. It is in fact a non-fluorescent chromophore.
Metabion offers Eclipse® in its DNA and RNA dual-labelled probes portfolio. Click here
| Eclipse® as 5' modification | |
|---|---|
| MW as 5' | 537.9 g/mol |
| Absorption range | 390-625 nm |
| Absorption maximum | 522 nm |
| Extinction coefficient (260nm) | 6.6 mM-1cm-1 |
| Eclipse® as 3' modification | |
|---|---|
| MW | 537.9 g/mol |
| Absorption range | 380-550 nm |
| Absorption maximum | 453 nm |
| Extinction coefficient (260nm) | 6.6 mM-1cm-1 |