C3 spacer
C3 spacer represents an effective way to block polymerase extension, when positioned at the 3’-end of an oligonucleotide. Additionally, it can prevent exonuclease-dependent oligo cleavage.
Metabion offers C3 spacer modification in its DNA and RNA portfolio. Click here
| Specifications | |
|---|---|
| MW | 138 g/mol |
K.W. Cradic, J.E. Wells, L. Allen, K.E. Kruekeberg, R.J. Singh, and S.K.G. Grebe. Substitution of 3'-phosphate cap with a carbon-based blocker reduces the possibility of fluorescence resonance energy transfer probe failure in real-time PCR assays. Clinical Chemistry.(2004); 50:1080–1082.