2’ F-A

2'-Fluoro-deoxyadenosine (2'-F-A) confers thermodynamical stability to an oligonucleotide during hybridization of the oligo with its target. When incorporated into an antisense, the duplex Tm increases by 1.8°C per 2'-F residue added, compared to the native antisense. In RNA oligos, it is suggestable to additionally phosphorothiolate the 2'-F RNA phosphodiester linkages, to increase the oligo resistance to RNase H.

Tmincrease 2 F A

metabion offers 2’ F-A in its standard RNA portfolio. Click here!

MW 331.2 g/mol
Extinction coefficient (260 nm) 15.4 mM-1cm-1


J.M. Layzer, A.P. McCaffrey, A.K. Tanner, Z. Huang, M.A. Kay, and B.A. Sullenger. In vivo activity of nuclease-resistant siRNAs. RNA (2004), 10 (5): 766-71.

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