2’ F-G
2'-Fluoro-deoxyguanosine (2'-F-C) confers thermodynamical stability to an oligonucleotide during hybridization of the oligo with its target. When incorporated into an antisense, the duplex Tm increases by 1.8°C per 2'-F residue added, compared to the native antisense. In RNA oligos, it is suggestable to additionally phosphorothiolate the 2'-F RNA phosphodiester linkages, to increase the oligo resistance to RNase H.
metabion offers 2’ F-G in its standard DNA portfolio. Click here!
| Specifications | |
|---|---|
| MW | 347.19 g/mol |
| Extinction coefficient (260 nm) | 13.7 mM-1cm-1 |
J.M. Layzer, A.P. McCaffrey, A.K. Tanner, Z. Huang, M.A. Kay, and B.A. Sullenger. In vivo activity of nuclease-resistant siRNAs. RNA (2004), 10 (5): 766-71.