5-Me-dC stands for 5-methyl deoxycytosine. When inserted in an oligonucleotide, 5-Me-dC increases the stability of the resulting duplex by raising the Tm of 1.3°C per 5-Me-dC residue added. Therefore, 5-Me-dC- modified oligos can hybridize more efficiently to their targets. Use of 5-Me-dC results in:

  • Strong-binding PCR primers
  • Reduction of inflammatory response when used in antisense oligos rich in CpG motives.
  • Allows DNA methylation studies.

 Tmincrease 5 Me dC

Metabion offers 5-Met-dC in its DNA and RNA portfolio. Click here

MW 303.21 g/mol
Extinction coefficient (260 nm) 5.7 mM-1cm-1


Y. Lebedev, N. Akopyants, T. Azhikina, Y. Shevchenko, V. Potapov, D. Stecenko, D. Berg, E. Sverdlov. Oligonucleotides containing 2-aminoadenine and 5-methylcytosine are more effective as primers for PCR amplification than their nonmodified counterparts. Genet Anal. (1996), 13: 15-21.

S.P. Henry, K. Stecker, D. Brooks, D. Monteith, B. Conklin, C.F. Bennett. Chemically modified oligonucleotides exhibit decreased immune stimulation in mice. J. Pharmacol. Exp. Ther. (2000), 292: 468-479.

T. Ishii, M. Fujishiro, M. Masuda, S. Teramoto, T. Matsuse. A methylated oligonucleotide induced methylation of GSTP1 promoter and suppressed its expression in A549 lung adenocarcinoma cells. Cancer Letters (2004), 212: 211-223.

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