metabion Black Quenchers: MBQ-1 and MBQ-2
MBQ-1 and MBQ-2 stand for metabion Black Quencher 1
and metabion Black Quencher 2.
MBQ-modifications consist of dark quenchers often used in dual-labelled probes for real-time PCR and are structurally identical to their respective BHQ-counterparts.
Dark Quenchers absorb energy without emitting
fluorescence. They are in fact non-fluorescent chromophores.
The MBQ-1 (Figure 1) dark quencher exhibits strong absorption between 480 nm and
580 nm, making it highly effective at quenching fluorophores that
fluoresce within this range, such as 6-Fam, TET, JOE, HEX, or a variety
of Atto dyes.
Figure 1. Structure of 3'-MBQ-1.
The absorbance spectra of MBQ-1 is identical to the absorbance spectra of BHQ-1 (Figure 2), which indicates that also the quenching range is identical.
For more details, please refer to our Dual-Labelled Probes (DLPs) and the corresponding Reporter-Quencher combination table.
MBQ-1 can quench fluorescence through both FRET and static quenching mechanisms.
Figure 2. Absorbance spectra of MBQ-1- and BHQ-1-labelled oligonucleotides. As visible, the absorbance max of MBQ-1 is 534 nm, exactly as its counterpart BHQ-1.
The MBQ-2 (Figure 3) dark quencher exhibits strong absorption between 550 nm and
650 nm, making it highly effective at quenching fluorophores that
fluoresce within this range, such as Tamra, ROX, or a variety
of Atto dyes.
Figure 3. Structure of 3'-MBQ-2.
The absorbance spectra of MBQ-2 is identical to the absorbance spectra of BHQ-2 (Figure 4), which indicates that also the quenching range is identical.
For more details, please refer to our Dual-Labelled Probes (DLPs) and the corresponding Reporter-Quencher combination table.
MBQ-2 can quench fluorescence through both FRET and static quenching mechanisms.
Figure 4. Absorbance spectra of MBQ-2- and BHQ-2-labelled oligonucleotides. As visible, the absorbance max of MBQ-2 is 579 nm, exactly as its counterpart BHQ-2.
metabion offers MBQ-1 and MBQ-2 at 3' in its DNA and RNA dual-labelled probes portfolio: