Polymerases & PCR mixes
Following the publication of the first paper about DNA polymerase in 1956 by the group of Arthur Kornberg, the use of DNA polymerases in cell-free environment made its way to become a routine technique in molecular biology at light speed. Nowadays, polymerase chain reaction (PCR) has become indispensable for a variety of applications, spanning from research to diagnostics and drug development.
Metabion will assist your experimental needs, with a PCR mixes and polymerases that fully complement our DNA/ RNA product portfolio.
Our service at a glance:
- You order your PCR mix/ polymerase. If you require assistance, you can always rely on our top quality Customer Service via chat, telephone or email.
- If you order within 6 pm German time, we start processing your order on the same day.
- Enzymes will be shipped as soon as possible after receiving your confirmed order. If you want to combine shipments of different product categories/orders for cost saving reasons, please indicate.
- Our default shipping mode is sending by Express service overnight at EURO 10,00 per shipment within Germany. If the value of your order is > EURO 250,00, shipping within Germany is free of charge!
- To ensure our enzymes maintain our high quality standards, all enzyme orders are shipped with cool packs.
Below you can find a complete list of our top-performing PCR mixes and polymerases.
| Product | Cat. No. | Amount | Product type | Amplification target |
|---|---|---|---|---|
| mi-Taq only | mi-E8001S mi-E8001L |
200 u 1000 u |
Taq polymerase with 3 different reaction buffers and MgCl2 stock solution | PCR templates up to 2 kb in length |
| mi-Taq Set | mi-E6003 | 500 u | Taq polymerase in a set with dNTPs and loading dye | PCR templates up to 2 kb in length |
| mi-Taq Mix | mi-E8016 | 200 rx | 2x PCR Master Mix with Taq polymerase | PCR templates up to 4 kb in length |
| mi-Red Load Taq Mix | mi-E7001S mi-E7001L |
100 rx 500 rx |
mi-Taq polymerase in a 5x master mix with a red dye for direct gel loading | PCR templates up to 2 kb in length |
| mi-Hot Taq | mi-E8009S mi-E8009L |
200 u 1000 u |
Hot Start Taq Polymerase for high specificity, antibody-blocked | For routine PCR applications (up to 4 kb in length), low-copy-number targets in complex backgrounds or when prolonged room-temperature set up is required, high throughput PCR or genotyping |
| mi-Hot Taq Mix | mi-E8011 | 100 rx | antibody-inhibited hot-start Taq polymerase in a 2x master mix | Best for real-time PCR |
| mi-Pfu DNA Polymerase | mi-E7022S mi-E7022L |
100 u 500 u |
Thermostable DNA pol. With 3’→5’ exonuclease (proofreading) activity | Polymerization reactions requiring high fidelity synthesis. 50 folds higher fidelity, compared to Taq pol. And 2 folds higher fidelity compared to standard Pfu |
| mi-1STEP RT-qPCR | mi-E8105 | 500 µl (2x conc.) | Mix of SCRIPT Reverse Transcriptase, Hot Start Polymerase, RNase Inhibitor, dNTPs, reaction buffer and stabilizers | Retro transcription of RNA into cDNA and amplification for regular PCR or qPCR targets |
| H Minus M-MLV Reverse Transcriptase | mi-E8103 | 10000 u | M-MuLV Rev. Transcriptase engineered to reduce RNAse H activity | cDNA synthesis, RNA analysis by primer extension, DNA labeling |
| mi-Modimerase | mi-E9000 | 200 u | Thermostable DNA pol. With 3’→5’ exonuclease (proofreading) activity | Optimized for Click Chemistry reagents and substrates |
| DNA Polymerase I Large Fragment (Klenow Fragment) | mi-E0148S mi-E0148L |
300 u 1500 u |
DNA Polymerase I with 3’→5’ exonuclease (proofreading) activity | Filling in receded 3' ends and digesting away protruding 3' overhangs of DNA fragments |
| Product | Cat. No. |
|---|---|
| mi-Taq Set | mi-E6003 |
| mi-Red Load Taq Mix | mi-E7001 |
| mi-Pfu DNA Polymerase | mi-E7022 |
| mi-Taq only | mi-E8001 |
| mi-Taq Mix | mi-E8016 |
| mi-Hot Taq | mi-E8009 |
| mi-Hot Taq Mix | mi-E8011 |
| mi-1STEP RT-qPCR | mi-E8105 |
| H Minus M-MLV Reverse Transcriptase | mi-E8103 |
| mi-Modimerase | mi-E9000 |
| DNA Polymerase I Large Fragment (Klenow Fragment) | mi-E0148 |
How to order
Online
Preferred ordering mode is using our web order portal for most convenient web shopping!
send an email to mi@mymetabion.com, include your customer id (if you have one).
If you are a new customer, please additionally provide us with
- Your shipping and billing address
- Any additional information that may be applicable, such as Purchase Order number, VAT number (VAT only for customers resident in the EU) etc
- and any special shipping requests.
Confirmation
Orders which indicate an email address will be confirmed by email.
Delivery
Our default shipping mode is sending by Express service overnight at EURO 10,00 per shipment within Germany. If the value of your order is > EURO 250,00, shipping within Germany is free of charge!
For other countries please inquire.
Note that Shipments on blue ice incur an additional charge of 4.50 EUR.
Shipments on dry ice incur an additional charge of 9.50 EUR.
Enzymes will be shipped as soon as possible after receiving your confirmed order. If you wish to combine shipments of different product categories/orders for cost saving reasons, please indicate this with your order.
To assure the quality of our enzymes, they are shipped on cool packs within Germany and Europe. If longer travelling times are expected (> 3 days, customs, etc.) dry ice can be used instead.
Transportation damages have to be immediately/ upon receipt of goods notified to the parcel service!
All our products are offered according to our terms & conditions. For in vitro use and research purposes only!